CleanCap Reagent M6, GMP

TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3' OMe) ], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superiorin vivoactivity compared to Cap-0 mRNAs produced by legacy capping methods.Design:The addition of a methyl group on position 6 of the first adenosine (m6A) may further increase protein expression relative toCleanCap AGorCleanCap AG (3'OMe) . It has been hypothesized that the m6A modification adjacent to the 7-methylguanosine cap can positively influence mRNA stability by preventing enzyme-mediated decapping (Mauer et al. 2017) .Usage:CleanCap M6 uses the same 5' AG initiation sequence as CleanCap AG and CleanCap AG (3'OMe) . For optimal results, use the Clean M6-specific IVT transcription buffer and the cap analog's concentration specified in the product insert. CleanCap M6 can be used in conjunction with TriLink'smodified and unmodified nucleotides, as well asIVT enzymes.Performance:The optimized standard-yield protocol produces >95% capping efficiency and typical crude yields of 4 to 5 mg/mL of mRNA fromin vitrotranscription (IVT) . The additional high-yield pulse-feed protocol is also included to provide up to 2x mRNA yields without impacting mRNA quality attributes.Availability:CleanCap M6 is available in "Research Use" grade as well as GMP-grade "For Further Processing." The GMP products are manufactured in accordance with ISO 9001:2015 and follow stringent quality standards, including highly controlled environments, thoroughly documented procedures, and full traceability of materials and processes. Find out more aboutCleanCap Reagent M6 >>