CleanCap M6 mCherry mRNA (N1MePsU), 100 ug

MCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found inDiscosoma sp.mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching. This mRNA is capped usingCleanCap® Reagent M6, TriLink's patented co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified withN1-methylpseudouridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA. CleanCap Reagent M6, otherwise known as CleanCap m6AG 3' OMe, produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA) . CleanCap M6 may further increase protein expression relative to previous generations of cap analogs, such as CleanCap AG or CleanCap AG (3' OMe), or mRNAs produced by enzymatic capping strategies. N1-methylpseudouridine is a modified uridine that can reduce immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression.