CleanCap® AU FLuc saRNA (5mC), 1 mg

This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the fireflyPhotinus pyralis, as self-amplifying RNA (saRNA) . FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin. Due to their self-amplification capability, saRNAs may provide more durable and longer protein expression than mRNAs. This ready-to-use saRNA construct is: Our catalog saRNA construct employs alphaviral replication machinery from Venezuelan equine encephalitis virus (VEEV) that encodes for the non-structural proteins (nsP1-4) . It is polyadenylated (26A) and optimized for mammalian systems.