CleanCap® AU EGFP saRNA (5mC), 5 x 1.00 mg

This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfishAequorea victoria, as self-amplifying RNA (saRNA) . EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm. Due to their self-amplification capability, saRNAs may provide more durable and longer protein expression than mRNAs. This ready-to-use saRNA construct is: Our catalog saRNA construct employs alphaviral replication machinery from Venezuelan equine encephalitis virus (VEEV) that encodes for the non-structural proteins (nsP1-4) . It is polyadenylated (26A) and optimized for mammalian systems.