BspQI, 500 U

BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang. BspQI’s recognition and cleavage sites: 5′ GGTCTCN↓ 3′3′ CGAGAAGNNNN↑ 5′ BspQI is thermostable and can be used in various molecular biology applications, including generation of linear DNA templates for in vitro transcription to synthesize RNA. The enzyme is provided 10 U/μL in storage buffer. It is also supplied with 1.25 mL of 10X RE Reaction Buffer (500 mM Tris,1000 mM NaCl, 100 mM MgCl2, 1 mg/mL rHSA, pH 7.5 at 25°C) . Unit definition: The amount of BspQI required to digest 1 μg of lambda DNA in 1 hour at 50°C in a 50 μL reaction.

Product Specifications

Volume

50 μL, 250 μL

Concentration

10 U/μL

Buffer

10 mM Tris, 100m M KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 g/L rHSA, 50% glycerol, pH 7.0 at 25°C

Applications Notes

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.