Bsal 5,000U

Bsal is a type IIS restriction enzyme that recognizes a specific DNA sequence (5′-GGTCTC-3′) and cleaves DNA outside of this recognition site, typically leaving 4-base overhangs. BsaI’s recognition and cleavage sites are:5′ GGTCTCN↓ 3′3′ CCAGAGNNNNN↑ 5′ BsaI is thermostable and can be used in various molecular biology applications, including generation of linear DNA templates forin vitrotranscription to synthesize RNA. Type IIS restriction enzymes bind to asymmetric DNA recognition sites but cut the DNA at defined positions located outside of those sites. The enzyme is provided 20 U/μL in storage buffer. It is also supplied with 1.25 mL of 10X RE Reaction Buffer (500 mM Tris, 1000 mM NaCl, 100 mM MgCl2, 1 mg/mL rHSA, pH 7.9 at 25°C) . Unit definition: The amount of BsaI required to digest 1 μg of pXba DNA in 1 hour at 37°C in a 50 μL reaction.

Product Specifications

Volume

50 μL, 250 μL

Host

E. coli

Applications

IVT template preparation, restriction digestion

Concentration

20 U/μL

Buffer

10 mM Tris, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5 g/L rHSA, 50% glycerol, pH 7.5 at 25°C

Shipping Conditions

Frozen

Storage Conditions

-15 to -25°C

Applications Notes

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.