2',3'-Dideoxythymidine-5'-Triphosphate

2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3' end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP) . This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University

Product Specifications

Applications

Aptamers

Concentration

100 mM

Purity

≥95% by AX-HPLC

Form

Li+

Buffer

H2O

Molecular Formula

C10H17N2O13P3 (free acid)

Molecular Weight

466.10 g/mole (free acid)

Shipping Conditions

Frozen

Storage Conditions

-20°C or below

Applications Notes

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.