Recombinant Thermus thermophilus Ribonuclease HII (rnhB)

Product Specifications

Product Name Alternative

RNase HII

Abbreviation

Recombinant Thermus thermophilus rnhB protein

Gene Name

RnhB

UniProt

Q5SLU5

Expression Region

1-203aa

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Target Sequence

MAEGPLEAPFWRKGLLVAGLDEAGRGAWAGPIVVGAVVLPPGEYPFRDSKLLSPKARERLAEKVKEVALAFALGVAEAAEVDRLGVLKATLLAAERALLSLPLAPEALVTDYLPLPTPLPLLSPPKADEKSPTVAAASILAKVHRDRIMDELDRLYPGYGFARHKGYGTQEHQEALLALGPSPVHRKRFAPVAQAPLRFPEAP

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

23.9 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.