Anti-Rad51 Antibody

Mouse Monoclonal Antibody specific to Rad51

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HsRAD51, hRAD51, RAD51 homolog A

Gene Name

RAD51

NCBI Gene ID

UniProt

Q06609

Cellular Locus

Nucleus, Cytoplasm, Cytoplasm, perinuclear region. Mitochondrion matrix, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein corresponding to full-length Rad51 (aa 1-338) of human Rad51 expressed in E. coli.

Target Antigen

DNA repair protein RAD51 homolog 1

Target

Rad51

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ICC/IF, IHC-P, IP, IHC, PLA

Field of Research

Cancer research

Purification Method

Purified by affinity on GST

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous rPubMed:12205100, PubMed:12442171, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:20413593, PubMed:23509288, PubMed:23754376, PubMed:26253028, PubMed:26681308, PubMed:28575658}.

Additionnal Information

Immunoblotting, <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive control: T24 bladder carcinoma cells and recombinant fusion protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human Rad51, a 37 kD protein that interacts with BRCA1. This interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact via the BRC repeats located with exon 11 of BRCA2. When these repeats are deleted, the interaction is lost, and cells become hypersensitive to DNA damage caused by methyl methanesulfonate (MMS).