AAV1-eGFP (N) -SD + AAV1-SA-eGFP (C)

Dual AAV-GFP controls include two AAV vectors, each carrying half of the GFP reporter. When co-delivered, homologous recombination restores the full-length mRNA, producing functional GFP. These controls provide a fast, visual proof-of-concept to validate dual-AAV co-delivery and recombination efficiency before moving to custom Dual AAV constructs.