AAV-Cre-GFP (AAV serotype 5) AAV (AAV5-Cre-GFP)

This AAV serotype 5 virus (with Capsid from AAV5 and ITR from AAV2) expresses Cre Recombinase (tagged with nuclear localization sequence) and eGFP marker. Each gene expression is driven by its own CMV promoter. Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.