CRISPR/Cas9 nuclease Adenovirus (Ad-Cas9)

The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR) -cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. This adenovirus expresses a codon-optimized NLS-spCas9 nuclease. It can be used along with guided RNA for genome-editing.