VWF Protein (C-His, Human)

Product Specifications

Abbreviation

VWF

Synonyms

F8VWF, VWD, VWF

Host

CHO

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized BDD-FVIII at 2 μg/mL (100 μL/well) can bind Recombinant Human vWF Protein (His Tag) (Cat: 10973-H08C), with a linear range of 0.16-4.0 μg/mL.

Function

Met1-Lys2813

Molecular Weight

The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.