VEGFC Protein (C-His, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his (Cat:10542-H08H) at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc (Cat:10806-H02H), the EC50 of VEGFR3 hFc (Cat:10806-H02H) is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 0.1-0.5 μg/mL. 4. Captured VEGFR3/FLT4 Protein, Human (Cat.No.10806-H02H) on anti-Human IgG Fc via CM5 Chip can bind VEGF-C (Cat.No.10542-H08H) with an affinity constant of 31.82 pM as determined in a SPR assay (Biacore T200) (Routinely tested) . 5.Immobilized Recombinant Human VEGF-C Protein (His Tag) (Cat: 10542-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human VEGFR3 / FLT4 Protein (ECD, Fc Tag) (Cat: 10806-H02H), the EC50 is 3-9 ng/mL (QC tested) .

The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.