VEGFA Protein (Native, Canine)

Product Specifications

Abbreviation

VEGFA

Host

HEK 293

Tag

Native

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Immobilized canine VEGF164 / VEGFA at 10 μg/ml (100 μl/well) can bind human VEGFR2-Fc (Cat:10012-H02H), The EC50 of human VEGFR2-Fc (Cat:10012-H02H) is 33.83-78.95 ng/ml. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 2-12 ng/mL.

Function

Met1-Arg190

Molecular Weight

The recombinant canine VEGF164 / VEGFA comprises 164 amino acids and has a predicted molecular mass of 19.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.