VCAM1 Protein (C-His, Mouse)

Product Specifications

Abbreviation

VCAM1

Synonyms

CD106, Vcam-1, Vcam1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells . When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT.

Function

Met1-Glu698

Molecular Weight

The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.