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TSHR Protein (C-His, Human)

Product Specifications

Abbreviation

TSHR

Synonyms

CHNG1, hTSHR-I, LGR3

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human TSHR Protein (His Tag) (Cat: 17299-H08B1) at 2 μg/mL (100 μl/well) on His Tag Antibody, Mouse MAb precoated (2 μg/mL, 100 μL/well) can bind Anti-TSHR (Research Grade K1-70 Biosimilar), the EC50 is 1-3 ng/mL (QC tested) . 2.Loaded Anti-TSHR (Research Grade K1-70 Biosimilar) on AR2G (Amine Reactive 2nd Gen) Biosensor, can bind Recombinant Human TSHR Protein (His Tag) (Cat. No. 17299-H08B1) with an affinity constant of 0.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Gly21-Leu260

Molecular Weight

The recombinant Human TSHR consists of 250 amino acids and predicts a molecular mass of 28.35 kDa. It migrates as approximately 35.7 kDa band in SDS-PAGE under reducing conditions.

Species

Human

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