TNFSF9 Protein (N-human IgG1-Fc, Rat)

1.Measured by its binding ability in a functional ELISA. Immobilized canine TNFRSF9-His (Cat:70095-D08H) at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9, The EC50 of rat Fc-TNFSF9 is 0.26-0.62 μg/ml. 2.Loaded Recombinant Rat 4-1BB Ligand/TNFSF9 Protein, hFc Tag (Cat. No. 80166-R01H) on proA Biosensor, can bind Recombinant Rat 4-1BB/CD137 Protein, His tag (Cat. No. 80176-R08H) with an affinity constant of 26.500 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant rat TNFSF9 comprises 463 amino acids and predicts a molecular mass of 50.9 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation.