TNFSF8 Protein (N-human IgG1-Fc, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized human CD30L-hFc (Cat: 10040-H01H) at 2 μg/ml (100 μl/well) can bind human CD30/TNFRSF8-Fch (Cat: 10777-H03H), the EC50 of human CD30/TNFRSF8-Fch is 15-60 ng/mL. 2.Immobilized Recombinant Human CD30 Ligand/TNFSF8 Protein (hFc Tag) (Cat: 10040-H01H) at 5 μg/mL (100 μL/well) can bind Recombinant Human CD30 / TNFRSF8 Protein (His & AVI Tag), Biotinylated (Cat: 10777-H49H-B), the EC50 is 26-78 ng/mL (Routinely tested) .

The recombinant human Fc/CD30L is a disulfide-linked homodimeric protein. The reduced monomer consists of 429 amino acids and has a predicted molecular mass of 47.8 kDa. As a result of glycosylation, the apparent molecular mass of rh Fc/CD30L monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions.