TNFSF15 Protein (N-His & AVI, Human)

Product Specifications

Abbreviation

TNFSF15

Synonyms

MGC129934, MGC129935, TL1, TL1A, TNLG1B, VEGI, VEGI192A

Host

HEK 293

Tag

N-His & AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A / TNFSF15 Protein (His & AVI Tag), Biotinylated (Cat: 17049-H56H2-B), the EC50 is 1-3 ng/mL. 2.Loaded Recombinant Human TL1A / TNFSF15 Protein, His Tag, Biotinylated, (Cat. No. 17049-H56H2-B) on SA Biosensor, can bind Recombinant Human DR3/TNFRSF25 Protein, hFc Tag (Cat. No. 12095-H02H) with an affinity constant of 2.57 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A / TNFSF15 Protein (His & AVI Tag), Biotinylated (Cat: 17049-H56H2-B), the EC50 is 22.1 ng/mL (Routinely tested) . 4.Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) captured on Protein A chip can bind Avi-tag Biotinylated Recombinant Human TL1A/TNFSF15 Protein (Trimer), His & AVI Tag (Cat. No. 17049-H56H2-B) with an affinity constant of 0.129 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Leu72-Leu251

Molecular Weight

The recombinant Human TL1A / TNFSF15 has a calculated molecular mass of 68.51 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 69.4 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 87.3 kDa verified by SEC-MALS.