TNFSF13B Protein (N-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 1 μg/mL (100 μl/well) can bind human TNFRSF17. The EC50 of human TNFRSF17 is 0.07 μg/mL. 2.Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human BAFF/BLyS/TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag, Biotinylated (Cat. No. 10620-H49H-B) with an affinity constant of 18.1 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Immobilized Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H) at 1 μg/mL (100 μL/well) can bind Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) (Cat: 10056-H01H), the EC50 is 1.0-4.0 ng/mL (QC tested) . 5.Immobilized Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) (Cat: 10056-H01H) at 1 μg/mL (100 μL/well) can bind Recombinant Human BAFFR / TNFRSF13C (Fc & AVI Tag), Biotinylated (Cat: 16079-H41H-B), the EC50 is 8-25 ng/mL (Routinely tested) .

The recombinant human BAFF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 389 amino acids and has a predicted molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhBAFF/Fc monomer is approximately 48-55 kDa due to glycosylation.