TNFSF12 Protein (N-mouse IgG1-Fc, Human, Cynomolgus)

Product Specifications

Abbreviation

TNFSF12

Host

HEK 293

Tag

N-mouse IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 4-16 ng/mL. 2. Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A (Cat:10431-H01H), The EC50 of human Fc-TNFRSF12A (Cat:10431-H01H) is 0.07-0.15 μg/ml.

Function

Ser94-His249

Molecular Weight

The recombinant human / cynomolgus TNFSF12 is a disulfide-linked homodimer. The reduced monomer comprises 392 amino acids and has a calculated molecular mass of 43.8 KDa. The apparent molecular mass of it is approximately 34 and 47 KDa respectively in SDS-PAGE.