TNFSF11 Protein (N-His, Mouse)

1.Immobilized Recombinant Human Osteoprotegerin / TNFRSF11B Protein (Fc Tag) (Cat: 10271-H02B) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse RANKL/TNFSF11 Protein (His Tag) (Cat: 50343-M07H), the EC50 is 20-60 ng/mL. 2.The bioactivity of mRANKL was determined by measuring the ability of mRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1-10ng/mL.

The recombinant Mouse TNFSF11 consisting of 169 amino acids and has a calculated molecular mass of 19.16 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 41.7 kDa and 23 kDa protein in SDS-PAGE under reducing conditions.