TNFSF11 Protein (N-rabbit IgG-Fc, Human)

Product Specifications

Abbreviation

TNFSF11

Synonyms

CD254, hRANKL2, ODF, OPGL, OPTB2, RANKL, sOdf, TNLG6B, TRANCE

Host

HEK 293

Tag

N-rabbit IgG-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human Osteoprotegerin / TNFRSF11B Protein (His Tag) (Cat: 10271-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human RANKL Protein (ECD, rFc Tag) (Cat: 11682-H15H1), the EC50 is 15-45 ng/mL. 2.The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL.

Function

Gly64-Asp245

Molecular Weight

The recombinant human TNFSF11 comprises 410 amino acids and has a predicted molecular mass of 46.18 kDa. The apparent molecular mass of the protein is approximately 134.21 kDa and 54.28 kDa in SDS-PAGE under reducing conditions due to glycosylation.