TNFSF11 Protein (N-mouse IgG1-Fc, Human)

Product Specifications

Abbreviation

TNFSF11

Synonyms

CD254, hRANKL2, ODF, OPGL, OPTB2, RANKL, sOdf, TNLG6B, TRANCE

Host

HEK 293

Tag

N-mouse IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF11B His (Cat:10271-H08H) at 2 μg/ml (100 μl/well) can bind Human RANKL mFc (Cat:11682-H04H), the EC50 of Human RANKL mFc is 15-80 ng/mL. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 15-45 ng/mL.

Function

Gly64-Asp245

Molecular Weight

The recombinant human TNFSF11/mFc comprises 418 amino acids and has a predicted molecular mass of 47.1 kDa. The apparent molecular mass of the monomer is approximately 50-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Species

Human