TNFRSF9 Protein (C-His, Mouse)

1.Immobilized Recombinant Mouse TNFRSF9 / CD137 / 4-1BB Protein (soluble form, His Tag) (Cat: 50811-M08H) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein (Fc Tag) (Cat: 50067-M01H), the EC50 is 6-20 ng/mL (QC tested) . 2.Loaded Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein, hFc Tag (Cat. No. 50067-M01H) on proA Biosensor, can bind Recombinant Mouse 4-1BB/CD137, His Tag (Cat. No. 50811-M08H) with an affinity constant of 0.497 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mouse Tnfrsf9 consists of 199 amino acids and predicts a molecular mass of 21.5 kDa. As a result of glycosylation, it migrates as an approximately 39 kDa band in SDS-PAGE under reducing conditions.