TNFRSF9 Protein (C-mouse IgG2a-Fc, Human)

Product Specifications

Abbreviation

TNFRSF9

Synonyms

4-1BB, CD137, CDw137, ILA, IMD109

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 0.05 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human CD137 / 4-1BB Protein (mFc Tag) (Cat: 10041-H38H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human 4-1BBL/TNFSF9 Protein (AVI & Fc Tag), Biotinylated (Cat: 15693-H42H-B), the EC50 is 1.5-4.5 ng/mL (Routinely tested) . 2.Loaded Recombinant Human 4-1BBL/TNFSF9 Protein (AVI & Fc Tag), Biotinylated (Cat. No. 15693-H42H-B) on ProA Biosensor, can bind Recombinant Human CD137 / 4-1BB Protein (ECD, mFc Tag) (Cat. No. 10041-H38H-UE) with an affinity constant of 19.2 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Gln186

Molecular Weight

The recombinant human TNFRSF9 consists of 401 amino acids and predicts a molecular mass of 44 kDa. As a result of glycosylation, it migrates as an approximately 58.9 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 104.1 kDa verified by SEC-MALS (Routinely tested) .