TNFRSF9 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

TNFRSF9

Synonyms

4-1BB, CD137, CDw137, ILA, IMD109

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human CD137 / 4-1BB Protein (ECD, Fc Tag) (Cat: 10041-H02H) at 0.1 μg/mL (100 μL/well) can bind Recombinant Human 4-1BBL/TNFSF9 Protein (AVI & Fc Tag), Biotinylated (Cat: 15693-H42H-B), the EC50 is 3-10 ng/mL (QC tested) . 2.Loaded Biotinylated Recombinant Human 4-1BB Ligand/TNFSF9 Protein, Fc Tag (Cat. No. 15693-H42H-B) on SA Biosensor, can bind Recombinant Human 4-1BB/CD137 Protein, hFc Tag (Cat. No. 10041-H02H) with an affinity constant of 4.29 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human CD137 / 4-1BB Protein (ECD, Fc Tag) (Cat: 10041-H02H) at 1 μg/mL (100 μL/well) can bind Anti-4-1BB (Research Grade Utomilu*mab Biosimilar), the EC50 is 8.9 ng/mL (Routinely tested) .

Function

Met1-Gln186

Molecular Weight

The recombinant human TNFRSF9 consists of 401 amino acids and predicts a molecular mass of 44 kDa. As a result of glycosylation, it migrates as an approximately 58.9 kDa band in SDS-PAGE under reducing conditions.

Species

Human