TNFRSF1B Protein (C-human IgG1-Fc, Mouse)

1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 ng/mL in the presence of 0.5 ng/mL of Recombinant Mouse TNFα (Routinely tested) . 2.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TNFR2 / CD120b / TNFRSF1B Protein (Fc Tag) (Cat: 50128-M02H), the EC50 is 8.5-25.5 ng/mL (Routinely tested) . 3.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein, Biotinylated (Cat: 50349-MNAE-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Recombinant Mouse TNFR2 / CD120b / TNFRSF1B Protein (Fc Tag) (Cat: 50128-M02H), the EC50 is 0.5-2.5 ng/mL (QC tested) .

The recombinant mouse TNFRSF1B/Fc is a disulfide-linked homodimer. The reduced monomer consists of 477 amino acids and has a predicted molecular mass of 52.3 kDa. In SDS-PAGE under reducing conditions, rm TNFRSF1B/Fc monomer migrates as an approximately 65 kDa band due to glycosylation.