TNFRSF1B Protein (C-human IgG1-Fc, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

TNFRSF1B

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-32 ng/mL in the presence of 1 ng/mL of cynoTNFα.

Function

Met1-Asp257

Molecular Weight

The recombinant cynomolgus / rhesus TNFRSF1B is a disulfide-linked homodimer. The reduced monomer comprises 476 amino acids and has a calculated molecular mass of 52.1 KDa.The apparent molecular mass of the protein is approximately 68 KDa respectively in SDS-PAGE.