TNFRSF1A Protein (C-human IgG1-Fc, Mouse)

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha (Cat:50349-MNAE) at 2 μg/ml (100 μl/well) can bind Mouse TNFRSF1AhFc (Cat:50496-M02H), the EC50 of Mouse TNFRSF1AhFc is 1.5-9.0 ng/mL. 2. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 15-65 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

The secreted recombinant mouse TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 amino acids and has a predicted molecular mass of 47.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A/Fc monomer is approximately 55 kDa due to glycosylation.