TNF Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

TNF

Synonyms

DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-5 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Fc Tag) (Cat: 10602-H01H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFR1/CD120a Protein (AVI & His Tag), Biotinylated (Cat: 10872-H27H-B), the EC50 is 1-3 ng/mL (QC tested) .