TNF Protein (Native, Cynomolgus, Rhesus)

1.Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2-20 pg/mL. 2.Anti-TNFa (Research Grade Adalim*mab Biosimilar) captured on Protein A chip can bind Recombinant Cynomolgus, Rhesus TNF-alpha Protein (Cat. No. 90018-CNAE) with an affinity constant of 0.201 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Immobilized Recombinant Cynomolgus/Rhesus/TNF-alpha/TNFA/TNFSF1A/Cachectin Protein (Cat: 90018-CNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 3.0 ng/mL (Routinely tested) . 4.Immobilized Recombinant Cynomolgus/Rhesus/TNF-alpha/TNFA/TNFSF1A/Cachectin Protein (Cat: 90018-CNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus TNFR2 / CD120b / TNFRSF1B Protein (Fc Tag) (Cat: 90102-C02H), the EC50 is 1.5-6.0 ng/mL (QC tested) .