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TNF Protein (C-His, Human)

Product Specifications

Abbreviation

TNF

Synonyms

DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant Human TNF-alpha Protein (His Tag) (Cat: 10602-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF1A Protein (Fc Tag) (Cat: 10872-H02H), the EC50 is 1-4 ng/mL (QC tested) . 2.Immobilized Recombinant Human TNF-alpha Protein (His Tag) (Cat: 10602-H08H) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Golim*mab Biosimilar), the EC50 is 1.0 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human TNF-alpha Protein (His Tag) (Cat: 10602-H08H) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 0.3 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human TNF-alpha Protein (His Tag) (Cat: 10602-H08H) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 0.6 ng/mL (Routinely tested) .

Function

Val77-Leu233

Molecular Weight

The recombinant Human TNF consists of 168 amino acids and predicts a molecular mass of 18.8 kDa. It migrates as approximately 19.1 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 64.8 kDa verified by SEC-MALS.

Species

Human

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