TNF Protein (Native, Rat)

Product Specifications

Abbreviation

TNF

Synonyms

RATTNF, Tnf, TNF-alpha, Tnfa, Tnfsf2

Host

E. coli

Tag

Native

Purity

> 93 % as determined by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-6 pg/ml.

Function

Leu80-Leu235

Molecular Weight

The recombinant rat TNFα consists of 157 amino acids and has a calculated molecular mass of 17.4 kDa. It migrates as an approximately 15.9 kDa band in SDS-PAGE under reducing conditions.