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TIMP1 Protein (C-His, Human)

Product Specifications

Abbreviation

TIMP1

Synonyms

CLGI, EPA, EPO, HCI, TIMP, TIMP-1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 (R&D Systems, Catalog # ES001) . The IC50 value is < 6 nM.

Function

Met1-Ala207

Molecular Weight

The secreted recombinant human TIMP1 comprises 195 amino acids with a predicted molecular mass of 22 kDa. As a result of glycosylation, rhTIMP1 migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.