TGFB1 Protein (Native, Human, Rhesus, Cynomolgus, Canine)

1.Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.01-0.1 ng/mL. 2.Human liver cancer organoids were cultured with FGF2 (Cat#10014-HNAE), HGF (Cat#10463-HNAS), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), TGFB1 (Cat#10804-HNAC), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 3.Western blot analysis of extracts from serum-starved Hela, untreated (line A) or treated with TGF beta 1 (10804-HNAC) (5 ng/mL, 120min; +) (line B), using Phospho-SMAD2 (Ser465, 467) rabbit monoclonal Antibody (Cat: 110472-R0010) at 1:1000 dilution (upper) or Anti-SMAD2 Antibody, Rabbit polyclonal (Cat: 50727-T58) at 1:100000 dilution (lower) (Routinely tested) .

The recombinant human / rhesus / cynomolgus / canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.