TF Protein (Native, Human)
Product Specifications
Abbreviation
TF
Synonyms
HEL-S-71p, PRO1400, PRO1557, PRO2086, TFQTL1
Host
HEK 293
Tag
Native
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) at 2 μg/mL (100 μL/well) can bind Recombinant Human TFR1/CD71 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 11020-H44H1-B), the EC50 is 30-90 ng/mL (QC tested) . 2.Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag) (Cat: 11020-H07H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) with an affinity constant of 63.500 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human TFR1/CD71 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 11020-H44H1-B) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) with an affinity constant of 75.510 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Immobilized Recombinant Human Transferrin protein (Cat: 11019-HNAH2) at 2 μg/mL (100 μL/well) can bind Recombinant Human Transferrin Receptor/TFRC Protein (His Tag), Biotinylated (Cat: 11020-H07H-B), the EC50 is 646.8 ng/mL (Routinely tested) .
Function
Val20-Pro698
Molecular Weight
The recombinant Human TF consists of 679 amino acids and predicts a molecular mass of 75.19 kDa. It migrates as an approximately 79.4 kDa band in SDS-PAGE under reducing conditions.
Species
Human
Curated Selection
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