TF Protein (C-human IgG1-Fc, Sus scrofa (Pig) )

Product Specifications

Abbreviation

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 88 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind human His-TFRC (cat: 11020-H07H) functional ELISA. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 μg/mL.

Function

Met1-Thr715

Molecular Weight

The secreted recombinant pig (Sus scrofa) transferrin/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 935 amino acids and has a predicted molecular mass of 103 kDa. It migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.