TF Protein (C-His, Human)

Product Specifications

Abbreviation

Synonyms

HEL-S-71p, PRO1400, PRO1557, PRO2086, TFQTL1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 15-150ng/mL (Routinely tested) . 2.Immobilized Recombinant Human Transferrin / TF Protein (His Tag) (Cat: 11019-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TFR1/CD71 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 11020-H44H1-B), the EC50 is 60.2 ng/mL (Routinely tested) .

Function

Met1-Pro698

Molecular Weight

The secreted recombinant human transferrin comprises 690 amino acids with a predicted molecular mass of 76.6 kDa. It migrates as an approximately 74 kDa band in SDS-PAGE under reducing conditions due to glycosylation.