TEK Protein (C-human IgG1-Fc, Rat)

Product Specifications

Abbreviation

TEK

Synonyms

Tie-2, Tie2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized S1h-3C-mANGPT2 (Cat:50298-M07H) at 10 μg/mL (100 μL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62μg/mL.

Function

Met4-Leu743

Molecular Weight

The recombinant rat TEK/Fc is a disulfide-linked homodimer. The reduced monomer comprises 962 amino acids and predicts a molecular mass of 108 kDa. The apparent molecular mass of the rat TEK/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to glycosylation.