TEK Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

TEK

Synonyms

CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Loaded Recombinant Human TIE-2 Protein, hFc Tag (Cat. No. 10700-H02H) on PROA Biosensor, can bind Recombinant Human Angiopoietin-2/ANGPT2 Protein, His Tag (Cat. No. 10691-H08H) with an affinity constant of 6.6 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2.Loaded Recombinant Human TIE-2 Protein (hFc Tag) (Cat. No. 10700-H02H) on proA Biosensor, can bind Biotinylated Recombinant Human Angiopoietin-2/ANGPT2 Protein (His & AVI Tag) (Cat. No. 10691-H27H1-B) with an affinity constant of 0.547 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Lys745

Molecular Weight

The recombinant Human TEK consists of 961 amino acids and predicts a molecular mass of 107.37 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 132.5 kDa protein in SDS-PAGE under reducing conditions.