TACSTD2 Protein (C-His-AVI, Human)

1.Loaded Anti-TROP2 (Research Grade Sacituzumab Biosimilar) on ProA Biosensor, can bind Recombinant Human TROP2 Protein, His Tag (Cat. No. 10428-H49H-B) with an affinity constant of 0.373nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Immobilized Anti-TROP2 (Research Grade Sacituzumab Biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B), the EC50 is 0.8-2.4 ng/mL (Routinely tested) . 3.Anti-TROP2 (Research Grade Datopotamab Biosimilar) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B) with an affinity constant of 16.900 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Immobilized Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Anti-TROP2 (Research Grade Datopotamab Biosimilar), the EC50 is 0.6-2.0 ng/mL (QC tested) .

The recombinant human TACSTD2 consists of 274 amino acids and predicts a molecular mass of 31.14 kDa. It migrates as an approximately 48.07 kDa band in SDS-PAGE under reducing conditions.