Spike Protein (C-human IgG1-Fc, SARS-CoV-2)
Product Specifications
Abbreviation
Spike
Host
HEK 293
Tag
C-human IgG1-Fc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Bioactivity
1.Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) (40591-V02H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 10-50 ng/mL. 2.Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Protein, Fc Tag (Cat. No. 40591-V02H) captured on Protein A chip can bind Recombinant Human ACE2 Protein, His Tag (Cat. No. 10108-H08H) with an affinity constant of 1.50 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .
Function
Val16-Arg685
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) consists of 908 amino acids and predicts a molecular mass of 101.7 kDa.
Species
SARS-CoV-2
Curated Selection
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