Spike Protein (C-His, SARS-CoV-2)
Product Specifications
Abbreviation
Spike
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
Bioactivity
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2 μg/mL (100 μL/well) can bind 2019-nCoV S1 (D614G) h (40591-V08H3), the EC50 of 2019-nCoV S1 (D614G) h (40591-V08H3) is 250-650 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) -His Recombinant Protein (Cat. No. 40591-V08H3) with an affinity constant of 57.7 nM as determined by Octet RED System.
Function
Val16-Arg685 (D614G)
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) -His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.41 kDa.
Species
SARS-CoV-2
Curated Selection
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