Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, K986P, V987P) -His Recombinant Protein (40589-V08B3), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, K986P, V987P) -His Recombinant Protein (40589-V08B3) is 450-900 ng/mL.

Function

Met1-Pro1213 (R683A, R685A, K986P, V987P)

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD (R683A, R685A, K986P, V987P), His tag) consists of 1221 amino acids and predicts a molecular mass of 135.37 kDa.