Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) -His (Cat:40589-V08B6), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) -His (Cat:40589-V08B6) is 200-800 ng/mL.

Function

Met1-Pro1213 (del (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His tag) consists of 1206 amino acids and predicts a molecular mass of 134.1 kDa.