Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.

Bioactivity

Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat:10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1+S2 trimer Protein (His & AVI Tag), Biotinylated (Cat:40589-V49H21-B), the EC50 is 40-120ng/mL (QC tested) .

Function

Met1-Ala1214

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) comprises 1263 amino acids and has a predicted molecular mass of 140.28 kDa. It migrates as an approximately 184.9 and 152.6 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 538.6 kDa verified by SEC-MALS.