Spike Protein (C-human IgG1-Fc, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind S3-2019-nCov-2-RBD (319R-541F, N501Y) -Fc (Cat:40592-V02H1), the EC50 of S3-2019-nCov-2-RBD (319R-541F, N501Y) -Fc (Cat:40592-V02H1) is 1.5-8.0 ng/mL.

Function

Arg319-Phe541 (N501Y)

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 462 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, it migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.