Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag) (Cat:40592-V08H86), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag) (Cat:40592-V08H86) is 3.0-15.0 ng/mL.

Function

Arg319-Phe541 (K417T, E484K, N501Y)

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.7 kDa. As a result of glycosylation, it migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.